Optimization of prostate cancer cell detection using multiplex tyramide signal amplification

Multiplex fluorescent in situ hybridization in zebrafish embryos using tyramide signal amplification.

One of the strengths of the zebrafish is the ease with which in situ hybridization can be performed to determine spatial and temporal patterns of gene expression in whole embryos. Thus far, colorimetric detection methods are mainly used for these analyses. Here we describe a fluorescent in situ hybridization (FISH) protocol for whole-mount zebrafish embryos using tyramide signal amplification (...

Tyramide Signal Amplification Strategies for Fluorescence Labeling

Signal Intensity of High B-value Diffusion-weighted Imaging for the Detection of Prostate Cancer

Background: Diffusion-weighted imaging (DWI) is a main component of multiparametric MRI for prostate cancer detection. Recently, high b value DWI has gained more attention because of its capability for tumor characterization. Objective: To assess based on histopathological findings of transrectal ultrasound (TRUS)-guided prostate biopsy as a reference, an...

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

In our efforts to determine the patterns of expression and subcellular localization of Drosophila RNAs on a genome-wide basis, and in a variety of tissues, we have developed numerous modifications and improvements to our original fluorescent in situ hybridization (FISH) protocol. To facilitate throughput and cost effectiveness, all steps, from probe generation to signal detection, are performed...

Automated 5-plex fluorescent immunohistochemistry with tyramide signal amplification using antibodies from the same species

Background Immunohistochemical (IHC) detection of multiple antigens on the same tissue section represents a major unmet technological need in research and clinical diagnostics. While primary antibodies from different species have been used with differently labeled species-specific secondary antibodies, quite often the appropriate combination of antibodies is not available. More recently, primar...